Evaluating the Surface Charge of C18 Stationary Phases
نویسندگان
چکیده
منابع مشابه
High-resolution peptide mapping separations with MS-friendly mobile phases and charge-surface-modified C18.
Ionic analytes, such as peptides, can be challenging to separate by reverse-phase chromatography with optimal efficiency. They tend, for instance, to exhibit poor peak shapes, particularly when eluted with mobile phases preferred for electrospray ionization mass spectrometry. We demonstrate that a novel charged-surface C18 stationary phase alleviates some of the challenges associated with rever...
متن کاملThe Effect of Bubble Surface Charge on Phonophoresis
It is over several decades that ultrasound is used to enhance the transdermal drug delivery (phonphoresis). The mechanism of the enhancement is not fully understood and the ability of ultrasound on the enhancement for some drugs is unclear. The effect of continuous wave 870 KHz ultrasound at intensity of 1 W/cm 2 for 15 minutes on transdermal absorption of piroxicam from solution and gel formu...
متن کاملA controlled thiol-initiated surface polymerization strategy for the preparation of hydrophilic polymer stationary phases.
A controlled thiol-initiated surface polymerization strategy has been successfully developed and employed to prepare hydrophilic polymer stationary phases, which exhibited excellent chromatographic performance and protein non-fouling properties.
متن کاملStructural insights into the effects of charge-reversal substitutions at the surface of horseradish peroxidase
Horseradish peroxidase (HRP), has gained significant interests in biotechnology, especially in biosensor field and diagnostic test kits. Hence, its solvent-exposed lysine residues 174, 232, and 241 have been frequently modified with the aim of improving its stability and catalytic efficiency. In this computational study, we investigated the effects of Lys-to-Glu substitutions on HRP structure t...
متن کاملPeptide Mapping and Small Protein Separations with Charged Surface Hybrid (CSH) C18 and TFA-Free Mobile Phases
■ ■ CSH130 C18 is QC tested with a tryptic digest of cytochrome c IN T RODUC T ION Peptide mapping of a biopharmaceutical, when employed for quality control, has traditionally involved detection by UV absorbance. However, to characterize the species in a peptide map, LC separations often must be coupled with ESI-MS. Mobile phases containing trifluoroacetic acid (TFA) have been almost exclusivel...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Chromatographic Science
سال: 2008
ISSN: 0021-9665,1945-239X
DOI: 10.1093/chromsci/46.1.45